Advantages: - Simplicity: The method is easy to perform and does not require sophisticated equipment. - Cost-Effective: It is relatively inexpensive compared to other antimicrobial susceptibility tests. - Standardization: Provides a standardized approach for comparing results across different laboratories.
Limitations: - Qualitative Results: The method provides qualitative rather than quantitative data on antibiotic susceptibility. - Influence of Variables: Factors such as agar depth, inoculum size, and incubation conditions can affect the results. - Not Suitable for All Bacteria: Some bacteria, such as anaerobes and fastidious organisms, may not grow well on standard media used in this method.
