1. Sample Collection: The process begins with the collection of a bacterial sample from the patient, which could be from a wound, blood, urine, or other sources. 2. Inoculation: The collected sample is then inoculated onto an agar plate. 3. Application of Antibiotic Disks: Antibiotic-impregnated disks are placed on the surface of the inoculated agar using sterile forceps. 4. Incubation: The plate is incubated at a specified temperature, usually 35-37°C, for 18-24 hours. 5. Measurement: After incubation, the zones of inhibition around the disks are measured in millimeters to determine the susceptibility of the bacteria to each antibiotic.
