Isolate the bacteria from the patient sample.
Spread the bacteria evenly on a
Mueller-Hinton agar plate.
Place antibiotic-impregnated disks on the agar surface.
Incubate the plate at 35-37°C for 18-24 hours.
Measure the
zone of inhibition around each disk.
How Can Nurses Utilize the Results?
Nurses can utilize the results of the Kirby Bauer Method to adjust
patient care plans. If the test indicates that the current antibiotic is ineffective, nurses can collaborate with physicians to switch to a more effective treatment. This is particularly important in critical care settings where timely and appropriate antibiotic therapy can significantly impact patient outcomes.
Use a
standardized procedure for sample collection and processing.
Ensure the use of fresh and properly stored
antibiotic disks.
Maintain appropriate incubation conditions.
Accurately measure and record the zones of inhibition.
Conclusion
The Kirby Bauer Method is a valuable tool in nursing for determining the
effective antibiotic treatment of bacterial infections. By understanding its application and limitations, nurses can play a critical role in managing infections and improving patient care outcomes.
