The process of primary cell culture involves several steps:
1. Isolation of Cells: Cells are isolated from a tissue sample, often through enzymatic digestion using enzymes like trypsin or collagenase. 2. Seeding: The isolated cells are then seeded into a culture medium that provides the necessary nutrients and conditions for cell growth. 3. Incubation: Cells are incubated under optimal conditions, usually in a CO2 incubator, to allow for cell attachment and proliferation. 4. Monitoring: The cell culture is regularly monitored for cell growth, contamination, and viability.
