The procedure for KOH preparation involves several steps:
Collect the Sample: Depending on the suspected infection site, collect skin scrapings, nail clippings, or vaginal discharge using sterile tools. Place on Slide: Transfer the sample to a clean glass slide. Add KOH Solution: Add a drop of 10-20% KOH solution to the sample on the slide. Cover Slip: Place a cover slip over the sample to spread the KOH evenly. Wait: Allow the preparation to sit for 5-10 minutes to let the KOH dissolve the keratin and other cellular material. Examine: Examine the slide under a microscope using low and high power magnification to identify fungal elements.
