The process of counterstaining involves several steps:
1. Preparation: The sample is first fixed and prepared on a slide. 2. Primary Staining: The primary stain is applied to highlight specific components of the specimen. 3. Washing: Excess primary stain is washed off. 4. Counterstaining: The counterstain is applied to provide contrast. 5. Final Washing and Observation: The slide is washed again to remove excess counterstain and then examined under a microscope.
