Several challenges can affect the accuracy of acid-fast staining: - Sample Quality: Poor-quality samples can lead to false-negative results. Nurses must ensure proper sample collection and handling. - Technical Expertise: The staining process requires technical precision. Inadequate staining or decolorization can lead to inaccurate results. - Interpretation: Differentiating between acid-fast and non-acid-fast organisms can be challenging. Proper training and experience are essential for accurate interpretation of the results.
