While specific protocols can vary, the general steps for performing a basic staining procedure are:
Fixation - The sample is fixed to a slide using heat or chemicals to preserve its structure. Staining - The appropriate dye is applied to the sample and allowed to incubate for a specified time. Rinsing - Excess stain is washed off using water or other solvents. Counterstaining - An additional stain may be applied to provide contrast. Microscopy - The stained sample is examined under a microscope to observe and document findings.
