1. Preparation: An agar plate is inoculated with the bacterial strain to be tested. 2. Application of Disks: Antibiotic disks are placed on the surface of the agar. 3. Incubation: The plate is incubated to allow bacterial growth and antibiotic diffusion. 4. Measurement: After incubation, the zones of inhibition are measured and compared to standardized charts to determine the bacteria's susceptibility to each antibiotic.
