1. Preparation of Antimicrobial Solutions: Serial dilutions of the antimicrobial agent are prepared in a broth medium. 2. Inoculation: The bacterial isolate is added to each dilution. 3. Incubation: The inoculated broths are incubated for a specified period, usually 18-24 hours. 4. Observation: The broths are examined for bacterial growth. The MIC is the lowest concentration where no visible growth is observed.
