Sample Collection: A sample from the infected area (e.g., blood, urine, or tissue) is collected and sent to the laboratory. Isolation of Pathogens: The sample is cultured to isolate the pathogen causing the infection. Exposure to Antibiotics: The isolated pathogen is exposed to various antibiotics to determine which one inhibits its growth. Interpretation of Results: Results are interpreted to identify susceptible, intermediate, and resistant antibiotics.