fluorescence in situ hybridization (fish)

How is FISH Performed?

The FISH procedure involves several key steps:
Sample Preparation: A sample, usually blood, bone marrow, or tissue, is collected from the patient.
Probe Hybridization: Fluorescent probes, which are short sequences of DNA or RNA, are designed to bind to specific chromosomal regions.
Denaturation and Hybridization: The sample DNA is denatured to single strands and then allowed to hybridize with the probes.
Visualization: The hybridized probes are visualized under a fluorescence microscope, allowing for the detection of genetic abnormalities.

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