Cell line authentication typically involves several methods:
1. Short Tandem Repeat (STR) Profiling: This is the gold standard for cell line authentication. It involves comparing the STR profile of a cell line to known profiles in databases. 2. Karyotyping: This method examines the number and structure of chromosomes in the cell line. 3. Isoenzyme Analysis: This technique checks for specific enzyme patterns that can confirm the species of the cell line. 4. DNA Barcoding: This involves sequencing specific regions of the DNA to confirm the cell line’s identity.
