How is the Kirby-Bauer Disk Diffusion Method Performed Using Mueller Hinton Agar?
The Kirby-Bauer disk diffusion method is a standardized technique for assessing bacterial susceptibility to antibiotics. The process involves:
Inoculating the Mueller Hinton Agar plate with a standardized bacterial suspension. Placing antibiotic-impregnated disks onto the surface of the agar. Incubating the plate to allow bacterial growth and antibiotic diffusion. Measuring the zones of inhibition around the disks to determine bacterial susceptibility.
